陈丽萍, 韩明明, 于功昌, 王世军. 清燥救肺汤对二氧化硅致大鼠肺损伤的抗炎作用研究[J]. 职业卫生与应急救援, 2020, 38(5): 437-442. DOI: 10.16369/j.oher.issn.1007-1326.2020.05.001
引用本文: 陈丽萍, 韩明明, 于功昌, 王世军. 清燥救肺汤对二氧化硅致大鼠肺损伤的抗炎作用研究[J]. 职业卫生与应急救援, 2020, 38(5): 437-442. DOI: 10.16369/j.oher.issn.1007-1326.2020.05.001
HEN Liping, HAN Mingming, YU Gongchang, WANG Shijun. Anti-inflammatory effect of Qingzaojiufei decoction on lung injury induced by SiO2 in rats[J]. Occupational Health and Emergency Rescue, 2020, 38(5): 437-442. DOI: 10.16369/j.oher.issn.1007-1326.2020.05.001
Citation: HEN Liping, HAN Mingming, YU Gongchang, WANG Shijun. Anti-inflammatory effect of Qingzaojiufei decoction on lung injury induced by SiO2 in rats[J]. Occupational Health and Emergency Rescue, 2020, 38(5): 437-442. DOI: 10.16369/j.oher.issn.1007-1326.2020.05.001

清燥救肺汤对二氧化硅致大鼠肺损伤的抗炎作用研究

Anti-inflammatory effect of Qingzaojiufei decoction on lung injury induced by SiO2 in rats

  • 摘要:
    目的 探讨清燥救肺汤干预对二氧化硅(SiO2)粉尘致大鼠肺组织损伤的抗炎作用。
    方法 将60只Wistar雄性大鼠随机分为阴性对照组,清燥救肺汤对照组,SiO2模型组和清燥救肺汤低、中、高剂量干预组,每组10只。经大鼠气管内灌注1 mL 50 mg/mL SiO2混悬液复制肺损伤动物模型,造模24 h后开始给予清燥救肺汤干预,清燥救肺汤低、中、高剂量及其对照组每日给药剂量分别为3.75、7.5、15、15 g/kg生药,阴性对照组与SiO2模型组以等体积生理盐水灌胃,1次/d,连续给药28 d。28 d后处死大鼠,取肺组织,行HE染色和Masson染色,碱水解法测定羟脯氨酸(HYP)含量,ELISA法测定大鼠肺组织TGF-β1、TNF-α、IL-1β、IL-10和IFN-γ细胞因子的水平。
    结果 组织病理学检查结果显示,3个清燥救肺汤干预组大鼠肺组织损伤程度均明显减轻。与SiO2模型组比较,清燥救肺汤各剂量组大鼠肺质量(湿重)和肺系数、肺组织中HYP含量、TGF-β1、TNF-α和IL-1β水平均出现不同程度的降低(P < 0.05),IL-10和IFN-γ水平均出现不同程度的升高(P < 0.05),且随着清燥救肺汤给药剂量的增加,其变化越明显。
    结论 清燥救肺汤可能通过抑制促炎因子TGF-β1、TNF-α、IL-1β,促进抗炎因子IL-10和IFN-γ的释放,减轻SiO2对大鼠肺组织的损伤作用。

     

    Abstract:
    Objective To explore the anti-inflammatory effect of Qingzaojiufei decoction (QD)on lung tissue damage induced by silicon dioxide(SiO2)dust in rats.
    Methods Totally 60 Wistar male rats were randomly divided into 6 groups, namely, negative control group(normal saline + normal saline), QD control groupnormal saline + QD(15 g/kg crude herb drugs), SiO2 treated groupsSiO2 treatment + saline and 3 QD intervention dose level(3.75, 7.5 and 15 g/kg crude herb drugs)groups, with 10 rats in each group. Rats were intratracheally given 1 mL of SiO2 suspension(50 mg/mL), or 1 mL normal saline(as control)to establish an animal model of lung injury. Then the rats were intragastrically administrated with QD or normal saline once a day, 24 h after the modeling, for 28 consecutive days. After 28 days, the rats were sacrificed, and the lung tissues were harvested. Then HE staining and Masson staining were performed, the content of hydroxyproline (HYP) was determined by alkaline hydrolysis method, and the cytokine levels of TGF-β1, TNF-α, IL-1β, IL-10 and IFN-γ were in rat lung tissues were determined by ELISA.
    Results Histopathological examination results showed that the damage of rat lung tissue in the three QD intervention groups was significantly alleviated. Compared with the SiO2 model group, the lung wet weight and lung coefficient, the HYP content and the levels of TGF-β1, TNF-α and IL-1β in the lung tissue of the rats in QD intervention groups were significantly reduced (P < 0.05), and the levels of IL-10 and IFN-γ increased in different degrees (P < 0.05), with dose-response relationship.
    Conclusions QD may reduce the damage of SiO2 to the lung tissue of rats by inhibiting the proinflammatory factors TGF-β1, TNF-α, IL-1β and promoting the release of anti-inflammatory factors IL-10 and IFN-γ.

     

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