Abstract: Objective To assess the status of perceived stress and occupational burnout among administrative staff in top tertiary hospitals in Guangzhou and to explore their correlation and influencing factors of occupational burnout. Methods In May 2022, administrative staff from six top tertiary hospitals in Guangzhou were surveyed with questionnaires of the Chinese Perceived Stress Scale (CPSS) and the Maslach Burnout Inventory-Human Services Survey (MBI-HSS). Spearman correlation analysis was used to examine the association between perceived stress and occupational burnout, and binary logistic regression models were applied to analyze potential factors influencing occupational burnout. Results A total of 120 individuals were surveyed, and 113 valid questionnaires were collected, with a valid response rate of 94.17%. Among these respondents, 62 individuals (54.87%) were identified as having health-risk levels of perceived stress; the median CPSS total score (P₂₅, P₇₅) was 26 (19, 28) points. Occupational burnout was detected among 53 individuals (46.90%), with a median MBI-HSS total score of 44 (25, 51) points. The CPSS total score, as well as the tension and loss-of-control dimensions, were all positively correlated with the MBI-HSS total score among this study population (r = 0.473, 0.278, and 0.320, respectively; all P < 0.01). Binary logistic regression analysis showed that administrative staff with health-risk levels of perceived stress had an increased risk of occupational burnout (OR = 8.781, 95%CI:3.472 to 22.206). Compared with those with low job liking, administrative staff who were very fond of their profession had a decreased risk of occupational burnout (OR = 0.237, 95%CI:0.064 to 0.876). Conclusions Administrative staff in top tertiary hospitals in Guangzhou experienced relatively high rates of perceived stress and occupational burnout. Hospital administrators should place a high priority on stress management for administrative staff and actively enhance their job liking to reduce the risk of occupational burnout and promote high-quality hospital development.